Non-enzymatic analysis of cholesterol in acetonitrile solutions

Non-enzymatic electrochemical study of cholesterol on conventional platinum electrode was carried out in acetonitrile solutions in the presence of potassium bromide by means of cyclic voltammetry and differential pulse voltammetry (DPV) techniques. The detection limit of platinum electrode towards i...

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Bibliographic Details
Published in:Physics and chemistry of liquids 2014-09, Vol.52 (5), p.601-607
Main Authors: Tsierkezos, Nikos G., Ritter, Uwe
Format: Article
Language:English
Subjects:
Acetonitrile
Cholesterol
Cholesterol oxidase
cyclic voltammetry
differential pulse voltammetry
Electrodes
Kinetics
Liquids
Platinum
platinum electrode
Potassium
potassium bromide
Solvents
Voltammetry
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Summary:Non-enzymatic electrochemical study of cholesterol on conventional platinum electrode was carried out in acetonitrile solutions in the presence of potassium bromide by means of cyclic voltammetry and differential pulse voltammetry (DPV) techniques. The detection limit of platinum electrode towards indirect oxidation of cholesterol (0.10 μM) determined by means of DPV in the concentration range of 0.032-0.143 mM appears to be significantly better compared to those estimated by means of other methods utilising cholesterol oxidase. The findings are very promising and clearly demonstrate that the indirect method used in the present work is quite suitable for the analysis of cholesterol in organic solvent media.
ISSN:0031-9104
1029-0451
DOI:10.1080/00319104.2014.890895