Post-Stress Thickening of Dextran/Concanavalin A Solutions Used as Sensitive Fluids in a Viscosimetric Affinity Assay for Glucose

Glucose‐dependent low‐stress and high‐stress rheology of a sensitive fluid (SF) containing high‐molecular‐weight dextran (30 g/L) and Concanavalin A (ConA, 10 g/L) was studied to define conditions for reproducible glucose determination by a viscosimetric sensor. The viscosity of the investigated SF...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology progress 1997, Vol.13 (6), p.722-726
Main Authors: Beyer, Uwe, Ehwald, Rudolf, Fleischer, Lutz-Günther
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
In solution. Condensed state. Thin layers
Methods. Procedures. Technologies
Molecular biophysics
Others
Physico-chemical properties of biomolecules
Various methods and equipments
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Glucose‐dependent low‐stress and high‐stress rheology of a sensitive fluid (SF) containing high‐molecular‐weight dextran (30 g/L) and Concanavalin A (ConA, 10 g/L) was studied to define conditions for reproducible glucose determination by a viscosimetric sensor. The viscosity of the investigated SF was reproducibly glucose‐dependent only at sufficient shear stresses (>0.5 Pa) . Rheometric parameters measured at low stresses (
ISSN:8756-7938
1520-6033
DOI:10.1021/bp970103u