Rhizobium leguminosarum strains forming nitrogen-rich nodules in Pisum sativum

• Published in: Folia microbiologica 1991-01, Vol.36 (2), p.177-182
• Main Authors: Skrdleta, V, Nemcova, M, Lisa, L, Kovarova, D, Novak, K
• Format: Article
• Language: English
• Subjects: activity; content; electron transfer; hydrogenase; nitrogen; nitrogen fixation; nodulation; nodules; Pisum sativum; Rhizobium leguminosarum
• ISSN: 0015-5632
• DOI: 10.1007/BF02814500

The nodules which developed on the roots of Pisum sativum after inoculation with Rhizobium leguminosarum bv. viciae strains showing a Hup super(+) symbiotic phenotype and/or a high relative efficiency of electron to dinitrogen (RE) had a high nitrogen content of their tissue. In comparison with the nodules initiated by a strain possessing the Hup super(-) symbiotic phenotype or by an indigenous soil Rhizobium) population, the nitrogen-rich root nodules contained up to four times more nitrogen (9.2% of dry mass). In the nitrogen-rich nodules, total amino acid, and especially Ala, Lys and Phe contents were significantly increased. The nitrogen-rich nodule symbiotic phenotype (Nrn) was well expressed, irrespective of pea cultivars and host plant age. If a strain showing the Nrn phenotype was applied in double-strain inocula, a significant correlation was found between increasing rates of the strain and increasing percentage nitrogen content of nodule tissue in the nodulated plants.