A nestin-negative precursor cell from the adult mouse brain gives rise to neurons and glia

Using a novel suspension culture approach, previously undescribed populations of neural precursor cells have been isolated from the adult mouse brain. Recent studies have shown that neuronal and glial precursor cells proliferate within the subependymal zone of the lateral ventricle throughout life,...

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Bibliographic Details
Published in:Glia 1997-12, Vol.21 (4), p.399-407
Main Authors: Kukekov, Valery G., Laywell, Eric D., Thomas, L. Brannon, Steindler, Dennis A.
Format: Article
Language:English
Subjects:
Animals
Antimetabolites - pharmacology
Biological and medical sciences
Brain - cytology
Brain - metabolism
brain marrow
Bromodeoxyuridine - pharmacology
Cell Transplantation
DNA Probes
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Glial Fibrillary Acidic Protein - metabolism
Graft Survival
Intermediate Filament Proteins - metabolism
Isolated neuron and nerve. Neuroglia
Mice
Mice, Inbred ICR
Mice, Transgenic
Microscopy, Electron
Nerve Tissue Proteins
Nestin
Neuroglia - physiology
Neuroglia - ultrastructure
Neurons - physiology
Neurons - ultrastructure
neuropoiesis
neurospheres
Rats
stem and progenitor cells
tissue culture
transplants
Vertebrates: nervous system and sense organs
Citations: Items that this one cites
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Summary:Using a novel suspension culture approach, previously undescribed populations of neural precursor cells have been isolated from the adult mouse brain. Recent studies have shown that neuronal and glial precursor cells proliferate within the subependymal zone of the lateral ventricle throughout life, and a persistent expression of developmentally regulated surface and extracellular matrix molecules implicates cell‐cell and cell‐substrate interactions in the proliferation, migration, and differentiation of these cells. By using reagents that may affect cell‐cell interactions, dissociated adult brain yields two types of cell aggregates, type I and type II spheres. Both sphere types are proliferative, and type I spheres evolve into type II spheres. Neurons and glia arise from presumptive stem cells of type II spheres, and they can survive transplantation to the adult brain. GLIA 21:399–407, 1997. © 1997 Wiley‐Liss, Inc.
ISSN:0894-1491
1098-1136
DOI:10.1002/(SICI)1098-1136(199712)21:4<399::AID-GLIA7>3.0.CO;2-Z