Neutrophil chemotactic activity and C5a following systemic activation of complement in rats

Using ELISA analysis, rat C5a was stimulated in serum from rats undergoing systemic activation of complement after intravenous infusion of purified cobra venom factor (CVF). Biological (neutrophil chemotactic) activity was also assessed. Serum levels of C5a were directly proportional to the amount o...

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Bibliographic Details
Published in:Inflammation 1997-06, Vol.21 (3), p.325-334
Main Authors: Schmid, E, Warner, R L, Crouch, L D, Friedl, H P, Till, G O, Hugli, T E, Ward, P A
Format: Article
Language:English
Subjects:
Animals
Antibodies - pharmacology
Chemotaxis, Leukocyte - drug effects
Complement Activation - drug effects
Complement C5a - antagonists & inhibitors
Complement C5a - metabolism
Elapid Venoms - toxicity
In Vitro Techniques
Kinetics
Lung - drug effects
Lung - immunology
Lung Injury
Macrophage-1 Antigen - metabolism
Male
Neutrophils - drug effects
Neutrophils - immunology
Rabbits
Rats
Up-Regulation
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Summary:Using ELISA analysis, rat C5a was stimulated in serum from rats undergoing systemic activation of complement after intravenous infusion of purified cobra venom factor (CVF). Biological (neutrophil chemotactic) activity was also assessed. Serum levels of C5a were directly proportional to the amount of CVF infused. C5a and neutrophil chemotactic activity, peaked by 5 min, then plateaued. In vitro addition of anti-C5a to serum samples of CVF-infused rats totally abolished chemotactic activity, indicating that all biological activity could be ascribed to C5a. Blood neutrophils obtained from CVF-infused animals showed a significant upregulation of CD11b, the increase being reduced (38%) in animals pretreated with anti-C5a. These findings indicate that infusion of CVF into rats produces generation of C5a, all chemotactic activity in serum being related to C5a. The in vivo generation of C5a is, at least inpart, responsible for upregulation of CD11b on blood neutrophils.
ISSN:0360-3997
DOI:10.1023/A:1027302017117