Chromatin protein HP1 interacts with the mitotic regulator borealin protein and specifies the centromere localization of the chromosomal passenger complex

Accurate mitosis requires the chromosomal passenger protein complex (CPC) containing Aurora B kinase, borealin, INCENP, and survivin, which orchestrates chromosome dynamics. However, the chromatin factors that specify the CPC to the centromere remain elusive. Here we show that borealin interacts dir...

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Bibliographic Details
Published in:The Journal of biological chemistry 2014-07, Vol.289 (30), p.20638-20649
Main Authors: Liu, Xing, Song, Zhenwei, Huo, Yuda, Zhang, Jiahai, Zhu, Tongge, Wang, Jianyu, Zhao, Xuannv, Aikhionbare, Felix, Zhang, Jiancun, Duan, Hequan, Wu, Jihui, Dou, Zhen, Shi, Yunyu, Yao, Xuebiao
Format: Article
Language:English
Subjects:
Amino Acid Motifs
Aurora Kinase B - genetics
Aurora Kinase B - metabolism
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell Division - physiology
Centromere - genetics
Centromere - metabolism
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - metabolism
Chromosomes, Human - genetics
Chromosomes, Human - metabolism
HEK293 Cells
HeLa Cells
Humans
Protein Structure, Tertiary
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Summary:Accurate mitosis requires the chromosomal passenger protein complex (CPC) containing Aurora B kinase, borealin, INCENP, and survivin, which orchestrates chromosome dynamics. However, the chromatin factors that specify the CPC to the centromere remain elusive. Here we show that borealin interacts directly with heterochromatin protein 1 (HP1) and that this interaction is mediated by an evolutionarily conserved PXVXL motif in the C-terminal borealin with the chromo shadow domain of HP1. This borealin-HP1 interaction recruits the CPC to the centromere and governs an activation of Aurora B kinase judged by phosphorylation of Ser-7 in CENP-A, a substrate of Aurora B. Consistently, modulation of the motif PXVXL leads to defects in CPC centromere targeting and aberrant Aurora B activity. On the other hand, the localization of the CPC in the midzone is independent of the borealin-HP1 interaction, demonstrating the spatial requirement of HP1 in CPC localization to the centromere. These findings reveal a previously unrecognized but direct link between HP1 and CPC localization in the centromere and illustrate the critical role of borealin-HP1 interaction in orchestrating an accurate cell division.
ISSN:1083-351X