Induction of hepatic CYP1A activity as a biomarker for environmental exposure to Aroclor 1254 in feral rodents

Specimens of the feral mouse species Reithrodontomys fulvescens trapped from a polychlorinated biphenyl (PCB)-contaminated field location had hepatic ethoxyresorufin (ETR) O-dealkylase activities and immunoreactive CYP1A protein contents which were two- to threefold higher than those measured in ani...

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Bibliographic Details
Published in:Archives of environmental contamination and toxicology 1992-04, Vol.22 (3), p.339-344
Main Authors: Lubet, R.A. (Frederick Cancer Research and Development Center, Frederick, MD), Nims, R.W, Beebe, L.E, Fox, S.D, Issaq, H.J, McBee, K
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Animal, plant and microbial ecology
Animals
Applied ecology
Aroclors - toxicity
BIFENILOS POLICLORADOS
Biological and medical sciences
Biomarkers
Body Burden
Carcinogens - toxicity
Chlorodiphenyl (54% Chlorine)
CONTAMINANTES
Cytochrome P-450 CYP1A1
Cytochrome P-450 Enzyme System - biosynthesis
Ecotoxicology, biological effects of pollution
Environmental Exposure
ENZYMIC ACTIVITY
Female
Fundamental and applied biological sciences. Psychology
HEPATITE
HEPATITIS
Liver - drug effects
Liver - enzymology
Male
Mice
Oxidoreductases - biosynthesis
POLLUANT
POLLUTANTS
POLYCHLORINATED BIPHENYLS
Polychlorinated Biphenyls - metabolism
POLYCHLORURE DE BIPHENYLE
Rats
Rats, Inbred F344
RODENTIA
Species Specificity
Terrestrial environment, soil, air
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Summary:Specimens of the feral mouse species Reithrodontomys fulvescens trapped from a polychlorinated biphenyl (PCB)-contaminated field location had hepatic ethoxyresorufin (ETR) O-dealkylase activities and immunoreactive CYP1A protein contents which were two- to threefold higher than those measured in animals of the same species and sex collected from non PCB-contaminated reference sites. Specimens with hepatic ETR O-dealkylase activities differing by as little as 50% could readily be assigned as originating from the PCB or reference sites by the use of a specific chemical inhibitor of cytochrome P450IA (CYP1A). The relative levels of ETR O-dealkylase activity in R. fulvescens significantly correlated with hepatic PCB burdens (r = 0.819, P less than 0.01). When the magnitudes of the induced ETR O-dealkylase activities corresponding to given hepatic PCB burdens were compared between the feral animals, F344/NCr rats (Rattus norvegicus) or B6C3F1 mice (Mus musculus) exposed in the laboratory to dietary Aroclor 1254, the order of sensitivity to the inducing effects of PCBs were F344/NCr rat greater than B6C3F1 mouse greater than R. fulvescens.
ISSN:0090-4341
1432-0703
DOI:10.1007/bf00212096