Conversion of D-carnitine into L-carnitine with stereospecific carnitine dehydrogenases

D-Carnitine was converted to L-carnitine by resting and permeabilized cells as well as with purified stereospecific carnitine dehydrogenases from Agrobacterium sp. With permeabilized cells only 11% of D-carnitine was converted into L-carnitine. Using highly stereospecific D- and L-carnitine dehydrog...

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Published in:Biotechnology letters 1997-07, Vol.19 (7), p.679-682
Main Authors: HANSCHMANN, H, KLEBER, H.-P
Format: Article
Language:English
Subjects:
Bacteria
Bacteriology
Biological and medical sciences
Cells
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
Permeability
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KLEBER, H.-P
description D-Carnitine was converted to L-carnitine by resting and permeabilized cells as well as with purified stereospecific carnitine dehydrogenases from Agrobacterium sp. With permeabilized cells only 11% of D-carnitine was converted into L-carnitine. Using highly stereospecific D- and L-carnitine dehydrogenases from Agrobacterium sp. (pH 8.5, 50 mM D-carnitine, 1 mM NAD + , 0.1 mM NADH, 25-fold excess of L-carnitine dehydrogenase) almost 50% of the D-carnitine could be converted into L-carnitine.[PUBLICATION ABSTRACT]
doi_str_mv 10.1023/A:1018399001074
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subjects Bacteria
Bacteriology
Biological and medical sciences
Cells
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
Permeability
title Conversion of D-carnitine into L-carnitine with stereospecific carnitine dehydrogenases
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