Characterization of the gene encoding the plastid-located glutamine synthetase of Phaseolus vulgaris: regulation of beta-glucuronidase gene fusions in transgenic tobacco

The gln-delta gene, which encodes the plastid-located glutamine synthetase of Phaseolus vulgaris, was cloned and its promoter region was sequenced. Primer extension analysis was used to map the two major transcription initiation sites which are about 90 nucleotides apart. A fusion of 2.3 kb of the u...

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Bibliographic Details
Published in:Plant molecular biology 1992-04, Vol.18 (6), p.1141-1149
Main Authors: Cock, J.M. (Warwick Univ., Coventry (United Kingdom). Dept. of Biological Sciences), Hemon, P, Cullimore, J.V
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
CODE GENETIQUE
CODIGO GENETICO
DNA Mutational Analysis
Fabaceae - enzymology
Fabaceae - genetics
Fundamental and applied biological sciences. Psychology
FUSION DE GENES
Gene Expression Regulation - physiology
GENE FUSION
Genes. Genome
GENETIC CODE
Glucuronidase - genetics
Glucuronidase - metabolism
Glutamate-Ammonia Ligase - genetics
Glutamate-Ammonia Ligase - metabolism
GLUTAMINA
GLUTAMINE
Light
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nicotiana - enzymology
Nicotiana - genetics
Nicotiana tabacum
Nitrogen - metabolism
PHASEOLUS VULGARIS
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants, Genetically Modified - enzymology
Plants, Genetically Modified - genetics
Plants, Medicinal
Plants, Toxic
Promoter Regions, Genetic - genetics
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
TRANSGENIC PLANTS
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Summary:The gln-delta gene, which encodes the plastid-located glutamine synthetase of Phaseolus vulgaris, was cloned and its promoter region was sequenced. Primer extension analysis was used to map the two major transcription initiation sites which are about 90 nucleotides apart. A fusion of 2.3 kb of the upstream region of the gln-delta gene to the reporter gene uidA encoding beta-glucuronidase was shown to be expressed in the chlorophyllous cell types of leaves and stems and in the root meristem region of transgenic tobacco. Analysis of a series of three 5' promoter deletion fusions revealed the presence of a region essential for promoter activity between -786 and -327 and regions involved in tissue-specific regulation and light regulation between -786 and +43.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00047717