Molecular analyses of the CMS-inducing 'Polima' cytoplasm in Brassica napus L

We report here on RFLP and Northern blot analyses of the "Polima" CMS-System in Brassica napus . Mitochondrial (mt)DNA and plastid (pt)DNA data indicate that the restorer, as well as the sterile and restored plants, possess a pol cytoplasm but that the restorer has no Brassica mt-plasmid....

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Bibliographic Details
Published in:Current genetics 1991-01, Vol.19 (4), p.323-327
Main Authors: Witt, U. (Hamburg Univ. (Germany). Inst. fuer Allgemeine Botanik. Arbeitsbereich Genetik), Hansen, S, Albaum, M, Abel, W.O
Format: Article
Language:English
Subjects:
'Polima' cytoplasm
Biological and medical sciences
BRASSICA NAPUS
CITOPLASMA
Classical genetics, quantitative genetics, hybrids
CMS
CYTOPLASM
CYTOPLASME
CYTOPLASMIC MALE STERILITY
ESTERILIDAD MASCULINA CITOPLASMICA
Fundamental and applied biological sciences. Psychology
GENE
GENES
GENETICA MITOCHONDRIAL
Genetics of eukaryotes. Biological and molecular evolution
GENETIQUE MITOCHONDRIALE
MITOCHONDRIAL GENETICS
Mitochondrial transcription
PLASMIDE
PLASMIDIOS
PLASMIDS
Pteridophyta, spermatophyta
STERILITE MALE CYTOPLASMIQUE
Vegetals
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Summary:We report here on RFLP and Northern blot analyses of the "Polima" CMS-System in Brassica napus . Mitochondrial (mt)DNA and plastid (pt)DNA data indicate that the restorer, as well as the sterile and restored plants, possess a pol cytoplasm but that the restorer has no Brassica mt-plasmid. An additional substoichiometric 3.3 kb EcoRI ptDNA fragment is present in the restorer. The transcription patterns of 12 mitochondrial-encoded genes of the pol cytoplasm have been determined for male-sterile and restored plants. Transcription patterns of the atp6 gene are correlated with the nuclear background. Restorer and restored plants exhibit two additional transcripts not detectable in sterile plants.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00355063