Seed-specific increased expression of 2S albumin promoter of sesame qualifies it as a useful genetic tool for fatty acid metabolic engineering and related transgenic intervention in sesame and other oil seed crops

• Published in: Plant molecular biology 2014-11, Vol.86 (4-5), p.351-365
• Main Authors: Bhunia, Rupam Kumar, Chakraborty, Anirban, Kaur, Ranjeet, Gayatri, T, Bhattacharyya, Jagannath, Basu, Asitava, Maiti, Mrinal K, Sen, Soumitra Kumar
• Format: Article
• Language: English
• Subjects: 2S Albumins, Plant - genetics; albumins; alpha-linolenic acid; alpha-Linolenic Acid - metabolism; beta-glucuronidase; Biochemistry; Biomedical and Life Sciences; Blotting, Western; crops; Crops, Agricultural - genetics; Fatty Acid Desaturases - genetics; Fatty acids; Fatty Acids - metabolism; Gene expression; Gene Expression Regulation, Plant; gene overexpression; genes; Genetic engineering; Glucuronidase - genetics; Glucuronidase - metabolism; Glycine max - genetics; Life Sciences; metabolic engineering; Metabolic Engineering - methods; Nicotiana - genetics; Oils & fats; oilseed crops; Plant biology; Plant Oils - metabolism; Plant Pathology; Plant Proteins - genetics; Plant Sciences; Plants, Genetically Modified; prediction; promoter regions; Promoter Regions, Genetic - genetics; Regulatory Elements, Transcriptional - genetics; Reverse Transcriptase Polymerase Chain Reaction; Seeds; Seeds - genetics; Seeds - metabolism; sesame oil; Sesamum - genetics; Sesamum - metabolism; Soybeans; stearoyl-CoA desaturase; temporal variation; tobacco; Transgenic plants
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/s11103-014-0233-6

The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin promoter. Prediction based deletion analysis of both the promoter elements confirmed the necessary cis-acting regulatory elements as well as the minimal promoter element for optimal expression in each case. The results also revealed that cis-regulatory elements might have been responsible for high level expression as well as spatio-temporal regulation of the sesame 2S albumin promoter. Transgenic over-expression of a fatty acid desaturase (fad3C) gene of soybean driven by 2S albumin promoter resulted in seed-specific enhanced level of α-linolenic acid in sesame. The present study, for the first time helped to identify that the sesame 2S albumin promoter is a promising endogenous genetic element in genetic engineering approaches requiring spatio-temporal regulation of gene(s) of interest in sesame and can also be useful as a heterologous genetic element in other important oil seed crop plants in general for which seed oil is the harvested product. The study also established the feasibility of fatty acid metabolic engineering strategy undertaken to improve quality of edible seed oil in sesame using the 2S albumin promoter as regulatory element.