Killer activity of Saccharomyces cerevisiae strains: partial characterization and strategies to improve the biocontrol efficacy in winemaking

• Published in: Antonie van Leeuwenhoek 2014-11, Vol.106 (5), p.865-878
• Main Authors: de Ullivarri, Miguel Fernández, Mendoza, Lucía M, Raya, Raúl R
• Format: Article
• Language: English
• Subjects: Antibiosis; Biochemical tests; Biological control; biological control agents; Biomedical and Life Sciences; Biopesticides; Chromosomes; DNA, Fungal - chemistry; DNA, Fungal - genetics; Ethanol; genes; growth retardation; Hydrogen-Ion Concentration; Killer Factors, Yeast - metabolism; Life Sciences; Medical Microbiology; Microbiology; Molecular Sequence Data; nitrogen; Original Paper; Pest Control, Biological; Plant Sciences; Polymerase Chain Reaction; Saccharomyces cerevisiae; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Sequence Analysis, DNA; Soil Science & Conservation; spoilage; starter cultures; Sulfur dioxide; Temperature; Toxins; Vitaceae; Wine - microbiology; winemaking; Wines; Yeast; Yeasts
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-014-0256-7

Killer yeasts are considered potential biocontrol agents to avoid or reduce wine spoilage by undesirable species. In this study two Saccharomyces cerevisiae strains (Cf8 and M12) producing killer toxin were partially characterized and new strategies to improve their activity in winemaking were evaluated. Killer toxins were characterized by biochemical tests and growth inhibition of sensitive yeasts. Also genes encoding killer toxin were detected in the chromosomes of both strains by PCR. Both toxins showed optimal activity and production at conditions used during the wine-making process (pH 3.5 and temperatures of 15–25 °C). In addition, production of both toxins was higher when a nitrogen source was added. To improve killer activity different strategies of inoculation were studied, with the sequential inoculation of killer strains the best combination to control the growth of undesired yeasts. Sequential inoculation of Cf8–M12 showed a 45 % increase of killer activity on sensitive S. cerevisiae and spoilage yeasts. In the presence of ethanol (5–12 %) and SO₂ (50 mg/L) the killer activity of both toxins was increased, especially for toxin Cf8. Characteristics of both killer strains support their future application as starter cultures and biocontrol agents to produce wines of controlled quality.