Saponarin from barley sprouts inhibits NF-κB and MAPK on LPS-induced RAW 264.7 cells

Saponarin (SA), a natural flavonoid, is known for its antioxidant and hepatoprotective activities. SA is the predominant compound (1142.7 ± 0.9 mg per 100 g) in barley sprouts, constituting 72% of the total polyphenol content. We investigated, for the first time, the effects of SA from barley sprout...

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Bibliographic Details
Published in:Food & function 2014-11, Vol.5 (11), p.3005-3013
Main Authors: Seo, Kyung Hye, Park, Mi Jin, Ra, Ji-Eun, Han, Sang-Ik, Nam, Min-Hee, Kim, Jin Hyo, Lee, Jin Hwan, Seo, Woo Duck
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents - pharmacology
Apigenin - analysis
Apigenin - pharmacology
Cell Line, Tumor
Cyclooxygenase 2 - genetics
Cyclooxygenase 2 - metabolism
Down-Regulation
Glucosides - analysis
Glucosides - pharmacology
Hordeum - chemistry
Hordeum vulgare
Interleukin-6 - genetics
Interleukin-6 - metabolism
Lipopolysaccharides - adverse effects
Macrophages - drug effects
Macrophages - metabolism
Mice
Mitogen-Activated Protein Kinases - genetics
Mitogen-Activated Protein Kinases - metabolism
NF-kappa B - genetics
NF-kappa B - metabolism
p38 Mitogen-Activated Protein Kinases - genetics
p38 Mitogen-Activated Protein Kinases - metabolism
Phosphorylation
Plant Extracts - pharmacology
Tandem Mass Spectrometry
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Summary:Saponarin (SA), a natural flavonoid, is known for its antioxidant and hepatoprotective activities. SA is the predominant compound (1142.7 ± 0.9 mg per 100 g) in barley sprouts, constituting 72% of the total polyphenol content. We investigated, for the first time, the effects of SA from barley sprouts on cellular anti-inflammatory responses. In lipopolysaccharide (LPS)-induced RAW 264.7 macrophages, SA suppressed the activation of NF-κB, as evidenced by the inhibition of NF-κB DNA binding, nuclear translocation, IκBα phosphorylation, and reporter gene expression, and it downregulated the expression of the pro-inflammatory mediator IL-6. Furthermore, SA reduced the transcription of NF-κB target molecules COX2 and FLIP inhibited the phosphorylation of mitogen-activated protein kinases ERK and p38. These results suggest that SA isolated from barley sprouts exerts anti-inflammatory effects in LPS-induced RAW 264.7 macrophages via inhibition of NF-κB, ERK and p38 signaling. Thus, SA may be a promising natural anti-inflammatory agent.
ISSN:2042-6496
2042-650X
DOI:10.1039/c4fo00612g