Characterization and immunogenicity in mice of recombinant influenza haemagglutinins produced in Leishmania tarentolae

Highlights • Six different haemagglutinin proteins from influenza were produced in L. tarentolae. • Expression and purification of the haemagglutinin proteins were easy. • Haemagglutinin proteins were immunogenic in mice. • L. tarentolae could be an alternative to influenza egg-based vaccines....

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Bibliographic Details
Published in:Vaccine 2014-09, Vol.32 (43), p.5570-5576
Main Authors: Pion, Corinne, Courtois, Virginie, Husson, Stéphanie, Bernard, Marie-Clotilde, Nicolai, Marie-Claire, Talaga, Philippe, Trannoy, Emanuelle, Moste, Catherine, Sodoyer, Régis, Legastelois, Isabelle
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antibodies, Viral - blood
Applied microbiology
Biological and medical sciences
Cloning, Molecular
Female
Fundamental and applied biological sciences. Psychology
Haemagglutinin
Hemagglutination Inhibition Tests
Hemagglutinin Glycoproteins, Influenza Virus - biosynthesis
Hemagglutinin Glycoproteins, Influenza Virus - immunology
Immunoglobulin G - blood
Influenza
Influenza A Virus, H1N1 Subtype
Influenza Vaccines - immunology
Lacertilia
Leishmania - metabolism
Leishmania tarentolae
Mice, Inbred BALB C
Microbiology
Orthomyxoviridae Infections - prevention & control
Recombinant Proteins - biosynthesis
Recombinant Proteins - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:Highlights • Six different haemagglutinin proteins from influenza were produced in L. tarentolae. • Expression and purification of the haemagglutinin proteins were easy. • Haemagglutinin proteins were immunogenic in mice. • L. tarentolae could be an alternative to influenza egg-based vaccines.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2014.07.092