Adjuvanted inactivated influenza A(H3N2) vaccines induce stronger immunogenicity in mice and confer higher protection in ferrets than unadjuvanted inactivated vaccines

• Published in: Vaccine 2014-09, Vol.32 (43), p.5730-5739
• Main Authors: Ann, Julie, Samant, Mukesh, Rheaume, Chantal, Dumas, Carole, Beaulieu, Edith, Morasse, Audrey, Mallett, Corey, Hamelin, Marie-Eve, Papadopoulou, Barbara, Boivin, Guy
• Format: Article
• Language: English
• Subjects: Adjuvants; Adjuvants, Immunologic - administration & dosage; Allergy and Immunology; Animals; Antibodies, Viral - blood; Applied microbiology; Biological and medical sciences; Cross Protection; Cysteine Endopeptidases - administration & dosage; Cytokines; Cytokines - immunology; Drug Combinations; Female; Ferrets; Fundamental and applied biological sciences. Psychology; H3N2 vaccine; Immune system; Immunity, Humoral; Immunity, Mucosal; Immunization; Immunogenicity; Immunoglobulin G - blood; Immunology; Infections; Influenza; Influenza A Virus, H3N2 Subtype; Influenza Vaccines - immunology; Laboratory animals; Lipopolysaccharides - administration & dosage; Male; Medical research; Mice; Mice, Inbred BALB C; Microbiology; Mustela; Orthomyxoviridae Infections - prevention & control; Respiratory tract; Studies; Th1 Cells - immunology; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Inactivated - immunology; Viruses
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2014.08.029

Highlights • Intramuscular injection of AS03- and AS25-adjuvanted vaccines generated a mixed Th1–Th2 response, predominantly a Th1 trend for AS25 and predominantly a Th2 trend for AS03 in mice. • Intranasal Protollin-adjuvanted vaccines induced a trend toward a more Th1 oriented response and a strong mucosal response in mice. • AS03- and AS25-adjuvanted vaccines induced protection against homologous and heterologous viral challenges in ferrets.