Altered extent of cross-linking of beta 1,6-glucosylated mannoproteins to chitin in Saccharomyces cerevisiae mutants with reduced cell wall beta 1,3-glucan content

The yeast cell wall contains beta 1,3-glucanase-extractable and beta 1,3-glucanase-resistant mannoproteins. The beta 1,3-glucanase-extractable proteins are retained in the cell wall by attachment to a beta 1,6-glucan moiety, which in its turn is linked to beta 1,3-glucan (J. C. Kapteyn, R. C. Montij...

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Bibliographic Details
Published in:Journal of bacteriology 1997-10, Vol.179 (20), p.6279-6284
Main Authors: Kaptieyn, J.C, Ram, A.F.J, Groos, E.M, Kollar, R, Montijn, R.C, Ende, H. van den, Llobell, A, Cabib, E, Klis, F.M
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
BETA GLUCANASA
BETA GLUCANASE
BETA-GLUCAN
CELL WALLS
CHEMICAL REACTIONS
CHITIN
CHITINE
ENZYMIC ACTIVITY
GLICOPROTEINAS
GLUCANE
GLUCANOS
GLUCANS
GLYCOPROTEINE
GLYCOPROTEINS
MANANOS
MANNANE
MANNANS
MUTANT
MUTANTES
MUTANTS
PARED CELULAR
PAROI CELLULAIRE
QUITINA
REACCIONES QUIMICAS
REACTION CHIMIQUE
RESISTANCE
SACCHAROMYCES CEREVISIAE
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Summary:The yeast cell wall contains beta 1,3-glucanase-extractable and beta 1,3-glucanase-resistant mannoproteins. The beta 1,3-glucanase-extractable proteins are retained in the cell wall by attachment to a beta 1,6-glucan moiety, which in its turn is linked to beta 1,3-glucan (J. C. Kapteyn, R. C. Montijn, E. Vink, J. De La Cruz, A. Llobell, J. E. Douwes, H. Shimoi, P. N. Lipke, and F.M. Klis, Glycobiology 6:337-345, 1996). The beta 1,3-glucanase-resistant protein fraction could be largely released by exochitinase treatment and contained the same set of beta 1,6-glucosylated proteins, including Cwp1p, as the beta 1,3-glucanase-extractable fraction. Chitin was linked to the proteins in the beta 1,3-glucanase-resistant fraction through a beta 1,6-glucan moiety. In wild-type cell walls, the beta 1,3-glucanase-resistant protein fraction represented only 1 to 2% of the covalently linked cell wall proteins, whereas in cell walls of fks1 and gas1 deletion strains, which contain much less beta 1,3-glucan but more chitin, beta 1,3-glucanase-resistant proteins represented about 40% of the total. We propose that the increased crosslinking of cell wall proteins via beta 1,6-glucan to chitin represents a cell wall repair mechanism in yeast, which is activated in response to cell wall weakening
ISSN:0021-9193
1098-5530
DOI:10.1128/JB.179.20.6279-6284.1997