Role of conserved and nonconserved residues in the Ca super(2+)-dependent carbohydrate-recognition domain of a rat mannose-binding protein: Analysis by random cassette mutagenesis

The carbohydrate-recognition domain of rat serum mannose-binding protein A has been subjected to random cassette mutagenesis. The results indicate that the precise arrangement of the regular portion of the domain containing the hydrophobic core is necessary for formation of a stable Ca super(2+)-lig...

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Bibliographic Details
Published in:The Journal of biological chemistry 1992-01, Vol.267 (15), p.10831-10841
Main Authors: Quesenberry, MS, Drickamer, K
Format: Article
Language:English
Subjects:
calcium
dependent
domains
mannose-binding protein
mutagenesis
rats
recognition
Online Access:Get full text
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Summary:The carbohydrate-recognition domain of rat serum mannose-binding protein A has been subjected to random cassette mutagenesis. The results indicate that the precise arrangement of the regular portion of the domain containing the hydrophobic core is necessary for formation of a stable Ca super(2+)-ligated structure under physiological conditions. The data also suggest that the saccharide-binding site is likely to be in close proximity to the bound Ca super(2+).
ISSN:0021-9258