Detection of the movement protein of red clover necrotic mosaic virus in a cell wall fraction from infected Nicotiana clevelandii plants

• Published in: Journal of general virology 1991-11, Vol.72 (11), p.2853-2856
• Main Authors: Osman, T. A. M, Buck, K. W
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; ATP-Binding Cassette Transporters; Base Sequence; Biological and medical sciences; Carrier Proteins - genetics; Cloning, Molecular; DNA, Viral; Electrophoresis, Polyacrylamide Gel; Escherichia coli - genetics; Escherichia coli Proteins; Fabaceae - microbiology; Fundamental and applied biological sciences. Psychology; Immunoblotting; Maltose-Binding Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mosaic Viruses - genetics; Nicotiana - microbiology; Nicotiana clevelandii; Phytopathology. Animal pests. Plant and forest protection; Plant viruses and viroids; Plants, Medicinal; Plants, Toxic; Recombinant Fusion Proteins - genetics; red clover necrotic mosaic virus; Systematics. Structure, properties and multiplication. Genetics; Viral Proteins - genetics
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-72-11-2853

Department of Biology, Imperial College of Science, Technology and Medicine, London SW7 2BB, U.K. The movement protein of red clover necrotic mosaic virus (RCNMV) was expressed in Escherichia coli as a fusion with a maltose-binding protein using the vector pMAL-cRI and used to produce an antiserum. The RCNMV movement protein was detected in a cell wall fraction obtained from infected Nicotiana clevelandii leaf tissue by immunoblotting using the movement protein antiserum. The movement protein could be detected 6 h after inoculation and reached a maximum after 24 h. In contrast, the virus capsid protein, detected in a soluble fraction by immunoblotting using a capsid antiserum, continued to increase for 72 h after inoculation. Received 5 June 1991; accepted 23 July 1991.