Analysis of structure and transcriptional activation of an osmotin gene

A Nicotiana tabacum gene encoding the basic PR-like protein osmotin was isolated and characterized. The gene is derived from the N. sylvestris parent of N. tabacum. In cell suspension cultures of tobacco, the osmotin gene was shown to be transcriptionally activated by treatment with ABA. Transcripti...

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Bibliographic Details
Published in:Plant molecular biology 1992-07, Vol.19 (4), p.577-588
Main Authors: Nelson, D.E. (Purdue Univ., West Lafayette, IN (USA). Dept. of Horticulture), Raghothama, K.G, Singh, N.K, Hasegawa, P.M, Bressan, R.A
Format: Article
Language:English
Subjects:
ABA
Abscisic Acid - physiology
ACIDE ABSCISSIQUE
ACIDO ABSCISICO
activation
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Blotting, Southern
Cells, Cultured
Cloning, Molecular
CODE GENETIQUE
CODIGO GENETICO
DNA
ETHYLENE
Ethylenes - pharmacology
ETILENO
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
genes
GENETIC CODE
Glucuronidase - genetics
Glucuronidase - metabolism
Kinetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nicotiana - genetics
NICOTIANA TABACUM
nucleotide sequence
Organ Specificity - genetics
osmotin
Plant Proteins - genetics
Plants, Toxic
predictions
Promoter Regions, Genetic
PROTEINAS
PROTEINE
PROTEINS
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Restriction Mapping
Single-Strand Specific DNA and RNA Endonucleases
transcription
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
Transformation, Genetic
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Summary:A Nicotiana tabacum gene encoding the basic PR-like protein osmotin was isolated and characterized. The gene is derived from the N. sylvestris parent of N. tabacum. In cell suspension cultures of tobacco, the osmotin gene was shown to be transcriptionally activated by treatment with ABA. Transcriptional activation of the osmotin promoter was further investigated in transformed plants carrying copies of a fusion of the cloned promoter to the beta-glucuronidase reporter gene. In these plants, the osmotin promoter is transcriptionally activated by the hormones ABA and ethylene. The sensitivity of the osmotin promoter to ABA applied exogenously decreased with age in both roots and shoots of young seedlings. NaCl shock also activated the promoter in plant tissues. The osmotin promoter is much more active in root tissues than in shoot tissues.
ISSN:0167-4412
1573-5028
DOI:10.1007/bf00026784