Nutritional factors regulating growth and accumulation of phenazine 1-carboxylic acid by Pseudomonas fluorescens 2-79

Pseudomonas fluorescens strain 2-79 (NRRL B-15 132) is a biocontrol agent of take-all of wheat caused by the fungus Gaeumannomyces graminis var. tritici. Strain 2-79 produces the antibiotic phenazine 1-carboxylic acid, which acts as the primary mechanism of disease suppression. As a first step towar...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1992-06, Vol.37 (3), p.388-392
Main Authors: Slininger, P.J, Jackson, M.A
Format: Article
Language:English
Subjects:
antibiotics
biological control
biosynthesis
growth rate
minerals
nutrient requirements
nutrition
phenazine 1-carboxylic acid
plant diseases and disorders
Pseudomonas fluorescens
roots
take-all
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Summary:Pseudomonas fluorescens strain 2-79 (NRRL B-15 132) is a biocontrol agent of take-all of wheat caused by the fungus Gaeumannomyces graminis var. tritici. Strain 2-79 produces the antibiotic phenazine 1-carboxylic acid, which acts as the primary mechanism of disease suppression. As a first step toward designing efficient methods of mass producing and formulating this biocontrol agent, the regulation of growth and antibiotic production by growth factors (including purines, pyrimidines, vitamins) and minerals (supplying B Ca2+, Co2+, Cu2+, Fe2+, Mg2+, Mn2+, Mo6+, Zn2+) was examined in defined liquid culture. Additions of boric acid and sulfates of iron and magnesium enhanced both cell and associated antibiotic accumulation. However, accumulation of the antibiotic alone improved with additions of zinc sulfate, ammonium molybdate, and cytosine, but worsened with addition of adenine. Interactive effects involving the sulfates of iron, magnesium, and zinc were observed, and optimal iron-magnesium and iron-zinc combinations were demonstrated with respect to biomass and antibiotic accumulation, respectively.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF00210998