The Agrobacterium tumefaciens vir gene transcriptional activator virG is transcriptional induced by acid pH and other stress stimuli

A set of Agrobacterium tumefaciens operons required for pathogenesis is coordinately induced during plant infection by the VirA and VirG proteins. The intracellular concentration of VirG increases in response to acidic media. We constructed a virG-lacZ transcriptional fusion. A strain containing thi...

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Bibliographic Details
Published in:Journal of bacteriology 1992-01, Vol.174 (4), p.1189-1196
Main Authors: Mantis, N J, Winans, S C
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
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Summary:A set of Agrobacterium tumefaciens operons required for pathogenesis is coordinately induced during plant infection by the VirA and VirG proteins. The intracellular concentration of VirG increases in response to acidic media. We constructed a virG-lacZ transcriptional fusion. A strain containing this fusion had higher levels of beta -galactosidase activity in acidic media than in media at neutral pH. We also pulse-labeled cellular proteins after a shift to low pH and detected several proteins whose synthesis was induced by these conditions. We conclude that P2 is primarily induced by acid pH and secondarily by certain other stimuli, each of which is stressful to cell growth.
ISSN:0021-9193