Identification and Characterization of a Conserved Family of Protein Serine/Threonine Phosphatases Homologous to Drosophila Retinal Degeneration C (rdgC)

The Drosophila retinal degeneration C (rdgC) gene encodes an unusual protein serine/threonine phosphatase in that it contains at least two EF-hand motifs at its carboxy terminus. By a combination of large-scale sequencing of human retina cDNA clones and searches of expressed sequence tag and genomic...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1997-10, Vol.94 (21), p.11639-11644
Main Authors: Sherman, Patti M., Sun, Hui, Macke, Jennifer P., Williams, John, Smallwood, Philip M., Nathans, Jeremy
Format: Article
Language:English
Subjects:
Alternative Splicing
Amino Acid Sequence
Amino acids
Animals
Antibodies
Biological Sciences
Caenorhabditis elegans - metabolism
Calcium-Binding Proteins
Complementary DNA
Conserved Sequence
Drosophila
Drosophila Proteins
Exons
Eyes & eyesight
Humans
Insects
Mammals
Mice
Molecular Sequence Data
Multigene Family
Neurology
Phosphatases
Phosphoprotein Phosphatases - biosynthesis
Phosphoprotein Phosphatases - chemistry
Photoreceptors
Phylogeny
Pineal Gland - metabolism
Proteins
Retina
Retina - metabolism
Retinal Degeneration
Retinal Rod Photoreceptor Cells - metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Worms
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Summary:The Drosophila retinal degeneration C (rdgC) gene encodes an unusual protein serine/threonine phosphatase in that it contains at least two EF-hand motifs at its carboxy terminus. By a combination of large-scale sequencing of human retina cDNA clones and searches of expressed sequence tag and genomic DNA databases, we have identified two sequences in mammals [Protein Phosphatase with EF-hands-1 and 2 (PPEF-1 and PPEF-2)] and one in Caenorhabditis elegans (PPEF) that closely resemble rdgC. In the adult, PPEF-2 is expressed specifically in retinal rod photoreceptors and the pineal. In the retina, several isoforms of PPEF-2 are predicted to arise from differential splicing. The isoform that most closely resembles rdgC is localized to rod inner segments. Together with the recently described localization of PPEF-1 transcripts to primary somatosensory neurons and inner ear cells in the developing mouse, these data suggest that the PPEF family of protein serine/threonine phosphatases plays a specific and conserved role in diverse sensory neurons.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.94.21.11639