Isolation and characterization of an afoA mutant of Bacillus subtilis

Abstract Among the Bacillus subtilis strains resistant to folic acid antagonists, afo mutants are phenotypically thymine independent and their dihydrofolate reductase, though expressed at normal levels, is still sensitive to these inhibitors. afo mutants isolated in our laboratory are all thymine in...

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Bibliographic Details
Published in:FEMS microbiology letters 1998-02, Vol.159 (2), p.227-232
Main Authors: Carcano, Clara, Barberio, Claudia, Carli, Alessio, Montorsi, Michela, Quarta, Claudio, Lorenzetti, Rolando
Format: Article
Language:English
Subjects:
Acid resistance
afoA
Antagonists
Antibiotics
Anti‐folate
Bacillus subtilis
Bacteriology
Biological and medical sciences
Complementation
Dihydrofolate reductase
Folic acid
Fundamental and applied biological sciences. Psychology
Genetic analysis
Genetic mapping
Hypersensitivity
Methotrexate
Microbiology
Mutants
Mutation
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Pyrithiamine
Reductases
Thiamine
Thymine
Trimethoprim
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Summary:Abstract Among the Bacillus subtilis strains resistant to folic acid antagonists, afo mutants are phenotypically thymine independent and their dihydrofolate reductase, though expressed at normal levels, is still sensitive to these inhibitors. afo mutants isolated in our laboratory are all thymine independent and methotrexate resistant over a wide range of concentrations but, like wild-type cells grown in the presence of thiamine, at high doses they return to show methotrexate sensitivity. They are all sensitive to trimethoprim, resistant to oxythiamine and show hypersensitivity to pyrithiamine. Though the phenotypic behavior of these afo mutants is very similar, genetic analysis suggests that they may belong to, at least, two different complementation groups. One of these mutations, for which we propose the name afoA, was mapped.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1998.tb12865.x