Serine tRNA complementary to the nonuniversal serine codon CUG in Candida cylindracea: evolutionary implications

In the asporogenic yeast Candida cylindracea, the codon CUG is read as serine instead of leucine. This is an unusual instance in which the amino acid assignment of a codon deviates from the universal code. To infer the evolutionary process of this change, the tRNA with the anticodon sequence CAG, wh...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1992-08, Vol.89 (16), p.7408-7411
Main Authors: Yokogawa, T. (Tokyo Institute of Technology, Yokohama, Japan), Suzuki, T, Ueda, T, Mori, M, Ohama, T, Kuchino, Y, Yoshinari, S, Motoki, I, Nishikawa, K, Osawa, S
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Anticodon
Anticodon - genetics
Base Sequence
Biochemistry
Biological and medical sciences
Biological Evolution
CANDIDA
Candida - genetics
Candida cylindracea
Classical genetics, quantitative genetics, hybrids
CODE GENETIQUE
CODIGO GENETICO
Codon - genetics
Codons
Evolution
Fundamental and applied biological sciences. Psychology
Genetic code
Genetic mutation
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Leucine - genetics
LEVADURA
LEVURE
Messenger RNA
Molecular Sequence Data
MUTACION
MUTATION
Nucleic Acid Conformation
NUCLEOTIDE
NUCLEOTIDOS
Protein Biosynthesis
Ribonucleic acid
RNA
RNA, Messenger - genetics
RNA, Transfer, Leu - genetics
RNA, Transfer, Ser - genetics
Serine - genetics
Splicing
Thallophyta, bryophyta
Transfer RNA
Vegetals
Yeasts
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Description
Summary:In the asporogenic yeast Candida cylindracea, the codon CUG is read as serine instead of leucine. This is an unusual instance in which the amino acid assignment of a codon deviates from the universal code. To infer the evolutionary process of this change, the tRNA with the anticodon sequence CAG, which is complementary to and thus responsible for translation of the codon CUG, has been identified. Indeed, this tRNA translates an in-frame CUG codon in a synthetic mRNA as serine in an in vitro translation system. The gene for the tRNA is interrupted by an intron in the anticodon loop. Sequence comparisons of the tRNA and its gene suggest that a single cytidine was inserted into the anticodon loop of the gene for tRNA(Ser)IGA during evolution to produce tRNA(Ser)CAG. The tRNA(Ser)CAG may be produced from its precursor molecule containing the cytidine insertion by splicing
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.89.16.7408