Multi-stage continuous high cell density culture systems: A review

A multi-stage continuous high cell density culture (MSC-HCDC) system makes it possible to achieve high productivity together with high product titer of many bioproducts. For long-term continuous operation of MSC-HCDC systems, the cell retention time and hydraulic retention time must be decoupled and...

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Bibliographic Details
Published in:Biotechnology advances 2014-03, Vol.32 (2), p.514-525
Main Authors: Chang, Ho Nam, Jung, Kwonsu, Choi, Jin-dal-rae, Lee, Joon Chul, Woo, Hee-Chul
Format: Article
Language:English
Subjects:
Bacteria
Biofuels
Biological and medical sciences
Bioreactors
Biotechnology
Cell Count
Cell Culture Techniques - instrumentation
Cell Culture Techniques - methods
Culture Media
Fermentation
Fundamental and applied biological sciences. Psychology
High cell density culture
Methods. Procedures. Technologies
Multi-stage continuous bioreactors
Product titer improvement
Productivity improvement
Recombinant Proteins - metabolism
Various methods and equipments
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Summary:A multi-stage continuous high cell density culture (MSC-HCDC) system makes it possible to achieve high productivity together with high product titer of many bioproducts. For long-term continuous operation of MSC-HCDC systems, the cell retention time and hydraulic retention time must be decoupled and strains (bacteria, yeast, plant, and animal cells) must be stable. MSC-HCDC systems are suitable for low-value high-volume extracellular products such as fuel ethanol, lactic acid or volatile fatty acids, and high-value products such as monoclonal antibodies as well as intracellular products such as polyhydroxybutyric acid (PHB), microbial lipids or a number of therapeutics. Better understanding of the fermentation kinetics of a specific product and reliable high-density culture methods for the product-generating microorganisms will facilitate timely industrialization of MSC-HCDC systems for products that are currently obtained in fed-batch bioreactors.
ISSN:0734-9750
1873-1899
DOI:10.1016/j.biotechadv.2014.01.004