Identification and expression of a novel MDM4 splice variant in human glioma

Abstract The product of the MDMX (or MDM4) gene is structurally related to the MDM2 oncoprotein and is also capable of interacting with the tumor suppressor protein p53. The MDM4 gene is overexpressed in several human tumors, while its product can be detected as various isoforms. This study was aime...

Full description

Saved in:
Bibliographic Details
Published in:Brain research 2013-11, Vol.1537, p.260-266
Main Authors: Wang, Xiaowen, Sheng, Ping, Guo, Xingzhong, Wang, Junyu, Hou, Lijun, Hu, Guohan, Luo, Chun, Dong, Yan, Lu, Yicheng
Format: Article
Language:English
Subjects:
Alternative splicing
Alternative Splicing - genetics
Biological and medical sciences
brain
carcinogenesis
Gene Expression Regulation, Neoplastic
gene overexpression
genes
genetic markers
Glioma
Glioma - genetics
Humans
MDM4
Medical sciences
mRNA
mutation
Mutation - genetics
neoplasms
Neurology
Nuclear Proteins - metabolism
oncogene proteins
p53
patients
Protein Isoforms - genetics
Proto-Oncogene Proteins - metabolism
quantitative polymerase chain reaction
RNA Splicing - genetics
RNA, Messenger - metabolism
Tumor Suppressor Protein p53 - genetics
Tumor Suppressor Protein p53 - metabolism
Tumors of the nervous system. Phacomatoses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract The product of the MDMX (or MDM4) gene is structurally related to the MDM2 oncoprotein and is also capable of interacting with the tumor suppressor protein p53. The MDM4 gene is overexpressed in several human tumors, while its product can be detected as various isoforms. This study was aimed to find the presence of aberrant mRNA transcripts of MDM4 in human glioma and their association with the clinicopathological characteristics of glioma patients. 42 glioma tissues were examined for MDM4 mRNA splicing variants by RT-PCR. A total of four distinct transcript sizes (full length-MDM4 851bp, MDM4-S 783bp, MDM4-A 701bp, MDM4-B 540bp) were detected. In the present study, we first report the novel alternative splicing form of MDM4, MDM4-B (GenBank accession no.KC479043.1). Expression of MDM4-B was present in various stages of human gliomas, but no significant correlation between presence of MDM4-B and malignancy of glioma was observed. The expression level of MDM4-B mRNA detected by real-time PCR was not only significantly associated with tumor stages, but also with p53 mutation and Ki-67 status which are important clinical molecular markers of glioma. Our data indicate that the novel variant MDM4-B may play a role in glioma tumorigenesis or cancer progression.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2013.07.054