Diversity analysis in Indian genotypes of linseed (Linum usitatissimum L.) using AFLP markers

• Published in: Gene 2014-10, Vol.549 (1), p.171-178
• Main Authors: Chandrawati, Maurya, Ramanuj, Singh, P.K., Ranade, S.A., Yadav, Hemant Kumar
• Format: Article
• Language: English
• Subjects: AFLP; Amplified Fragment Length Polymorphism Analysis; Analysis of Variance; Cluster Analysis; DNA Primers; Flax; Flax - classification; Flax - genetics; Genes, Plant; Genetic diversity; Genetic Markers; Genetic Variation; Genome, Plant; Genotype; Jaccard's coefficient; Linum usitatissimum; Plant Leaves - genetics; Polymorphism information content; Polymorphism, Restriction Fragment Length
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2014.07.067

AFLP fingerprinting of 45 Indian genotypes of linseed was carried out to determine the genetic relationship among them. Sixteen primer combinations produced 1142 fragments with 1129 as polymorphic and 13 as monomorphic fragments. Polymorphic fragments varied from 44 (E-ACA/M-CTA) to 94 (E-AGC/M-CAC) with an average of 70.6 fragments per primer combination. The frequency of polymorphism varied from 93.7% to 100% with an average of 98.8% across all the genotypes. The PIC value ranged from 0.19 to 0.31 with an average of 0.23 per primer combination. The primer pair E-AGC/M-CAC showed the maximum PIC value (0.31) followed by E-AGC/M-CAG (0.29), E-AAC/M-CAG (0.26) and E-AGC/M-CTA (0.25). Resolving power (RP) and marker index (MI) varied from 13.73 to 43.50 and 8.81 to 28.91 respectively. The Jaccard's similarity coefficient varied from 0.16 to 0.57 with an average of 0.26±0.05. The maximum genetic similarities (57%) were detected between genotypes Him Alsi-1 and Him Alsi-2, followed by Him Alsi-1 and GS41 and GS41 and LC-54. The genotypes R-552, Himani, RKY-14, Meera, Indira Alsi-32 and Suyog were found to be more divergent genotypes. The NJ clustering grouped all the 45 genotypes into three major clusters. In general the genotypes of cluster III had high oil content and those of cluster I had low oil content. At the population level, within population variance was much higher than between populations variance. •AFLP fingerprinting of 45 Indian genotypes of linseed was done.•Sixteen AFLP primers produced 1129 polymorphic fragments varying from 44 to 94 per primers.•PIC value varied from 0.19 to 0.31 and genetic similarity from 0.16 to 0.57 with an average of 0.26±0.05.•All the 45 genotypes clustered into three major groups based on NJ clustering and variance within population was higher than between populations.