Light-mediated control of gene expression in filamentous fungus Trichoderma reesei

We developed a light-mediated system based on synthetic light-switchable transactivators. The transactivators bind promoter upon blue-light exposure and rapidly initiate transcription of target transgenes in filamentous fungus Trichoderma reesei. Light is inexpensive to apply, easily delivered, and...

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Bibliographic Details
Published in:Journal of microbiological methods 2014-08, Vol.103, p.37-39
Main Authors: Wang, Wei, Shi, Xiang-Yu, Wei, Dong-Zhi
Format: Article
Language:English
Subjects:
Gene Expression
Gene Expression Regulation, Fungal - radiation effects
Gene Order
Genes, Reporter
Genetic Vectors - genetics
Hypocrea jecorina
Light
Light-mediated system
Light-switchable transactivator
Trans-Activators - metabolism
Trichoderma - genetics
Trichoderma - metabolism
Trichoderma - radiation effects
Trichoderma reesei
Vivid
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Summary:We developed a light-mediated system based on synthetic light-switchable transactivators. The transactivators bind promoter upon blue-light exposure and rapidly initiate transcription of target transgenes in filamentous fungus Trichoderma reesei. Light is inexpensive to apply, easily delivered, and instantly removed, and thus has significant advantages over chemical inducers. •For the first time, we describe a light-mediated system in Trichoderma reesei.•Our light-mediated system is orthogonal to internal cellulase system of T. reesei.•Our light-mediated system is useful in controlling gene expression in T. reesei.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2014.05.017