Paenibacillus abyssi sp. nov., isolated from an abyssal sediment sample from the Indian Ocean

• Published in: Antonie van Leeuwenhoek 2014-12, Vol.106 (6), p.1089-1095
• Main Authors: Huang, Xiao-Fang, Wang, Fa-Zuo, Zhang, Wei, Li, Jie, Ling, Juan, Yang, Jian, Dong, Jun-De, Tian, Xin-Peng
• Format: Article
• Language: English
• Subjects: bacteria; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; chemotaxonomy; Cluster Analysis; correlation; Cytosol - chemistry; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; fatty acids; Fatty Acids - analysis; Geologic Sediments - microbiology; Gram-positive bacteria; Hydrogen-Ion Concentration; Indian Ocean; Life Sciences; Medical Microbiology; Microbiology; Microscopy, Electron, Transmission; Molecular Sequence Data; new species; nucleotide sequences; Original Paper; Paenibacillus; Paenibacillus - classification; Paenibacillus - genetics; Paenibacillus - isolation & purification; Paenibacillus - physiology; Phylogenetics; Phylogeny; Plant Sciences; Quinones - analysis; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sediment samplers; Sediments; Sequence Analysis, DNA; sequence homology; Sodium chloride; Sodium Chloride - metabolism; Soil Science & Conservation; Taxonomy; Temperature
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-014-0277-2

A Gram-positive, rod-shaped bacterium, designated strain SCSIO N0306ᵀ, was isolated from an abyssal sediment sample collected from the Indian Ocean. The isolate was found to grow optimally at 0–2 % (w/v) NaCl, pH 7.0 and 30 °C. Comparative analysis of the 16S rRNA gene sequence showed that the isolate SCSIO N0306ᵀ belongs phylogenetically to the genus Paenibacillus, and to be most closely related to P. algorifonticola XJ259ᵀ (with 95.47 % sequence similarity), sharing less than 95.0 % sequence similarity with all other taxa of this genus. Chemotaxonomic analysis revealed MK-7 as the major isoprenoid quinone, the DNA G+C content was determined to be 45.5 mol%, and anteiso-C₁₅:₀, C₁₆:₀, and iso-C₁₅:₀ were identified as the major fatty acids. On the basis of this polyphasic taxonomic data, isolate SCSIO N0306ᵀ is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus abyssi sp. nov. is proposed. The type strain is SCSIO N0306ᵀ (= DSM 26238ᵀ = CGMCC 1.12987ᵀ).