Long-term muscle-derived cell culture: multipotency and susceptibility to cell death stimuli

Improvement in the yield of adult organism stem cells, and the ability to manage their differentiation and survival potential are the major goals in their application in regenerative medicine and in the adult stem cell research. We have demonstrated that adult rabbit muscle‐derived cell lines with a...

Full description

Saved in:
Bibliographic Details
Published in:Cell biology international 2013-04, Vol.37 (4), p.292-304
Main Authors: Kalvelyte, Audrone, Krestnikova, Natalija, Stulpinas, Aurimas, Bukelskiene, Virginija, Bironaite, Daiva, Baltriukiene, Daiva, Imbrasaite, Ausra
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Cell Culture Techniques
cell death
Cell Differentiation
Cell Line
Cell Survival
Chromones - pharmacology
differentiation
Morpholines - pharmacology
multipotency
Muscle, Skeletal - cytology
muscle-derived stem cell lines
Proto-Oncogene Proteins c-akt - metabolism
Rabbits
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Improvement in the yield of adult organism stem cells, and the ability to manage their differentiation and survival potential are the major goals in their application in regenerative medicine and in the adult stem cell research. We have demonstrated that adult rabbit muscle‐derived cell lines with an unlimited proliferative potential in vitro can differentiate into myogenic, osteogenic, adipogenic and neurogenic lineages. Studies of cell survival in vitro showed that differentiated cells, except neurogenic ones, are more resistant to apoptosis inducers compared to proliferating cells. Resistance to death signals correlated with the level of protein kinase AKT phosphorylation. Skeletal muscle‐derived cell lines can be multipurpose tools in therapy. Enhanced resistance of differentiated cells to certain types of damage shows their potential for long‐term survival and maintenance in an organism. This article was published online on 29 January 2013. An error was subsequently identified. This notice is included in the online and print versions to indicate that both have been corrected 6 March 2013.
ISSN:1065-6995
1095-8355
DOI:10.1002/cbin.10036