Characterization and phylogenetic diversity of Agrobacterium vitis from Serbia based on sequence analysis of 16S-23S rRNA internal transcribed spacer (ITS) region

Serious outbreaks of grapevine crown gall disease were observed in major Serbian viticultural regions during the last five years. Tumorigenic Agrobacterium vitis was identified as a causal agent by using conventional bacteriological and molecular tests. The 36 studied strains of A. vitis showed homo...

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Bibliographic Details
Published in:European journal of plant pathology 2014-12, Vol.140 (4), p.757-768
Main Authors: Kuzmanovic, Nemanja, Ivanovic, Milan, Prokic, Anelka, Gasic, Katarina, Zlatkovic, Nevena, Obradovic, Aleksa
Format: Article
Language:English
Subjects:
Agriculture
Agrobacterium vitis
Biomedical and Life Sciences
Crop diseases
crown galls
Deoxyribonucleic acid
DNA
Ecology
Genetic diversity
genetic variation
Helianthus
Helianthus annuus
internal transcribed spacers
Life Sciences
Lycopersicon esculentum
octopine
Phylogenetics
phylogeny
Plant Pathology
Plant Sciences
Plasmids
polymerase chain reaction
Rhizobium vitis
ribosomal RNA
sequence analysis
Tomatoes
Vitaceae
viticulture
Vitis
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Summary:Serious outbreaks of grapevine crown gall disease were observed in major Serbian viticultural regions during the last five years. Tumorigenic Agrobacterium vitis was identified as a causal agent by using conventional bacteriological and molecular tests. The 36 studied strains of A. vitis showed homogeneous biochemical and physiological characteristics, but were heterogeneous in their pathogenic properties, especially on tomato and sunflower. Furthermore, genetic differences related to chromosomal and plasmid DNA were observed. The Ti plasmid of 35 strains was classified as the octopine/cucumopine (O/C) type, whereas one was classified as the vitopine (V) type. The O/C strains were further divided into O/C-1 and O/C-2 groups based on PCR analysis. Moreover, the sequence analysis of the 16S-23S rRNA ITS region provided robust and precise delineation of studied strains. Although a high level of genetic diversity in A. vitis strains from Serbia was revealed by using this approach, their genotypic relatedness with the strains from other countries suggested their common origin. Also, association between the chromosomal and plasmid DNA was determined for some phylogenetic groups and clusters.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-014-0507-5