Functional involvement of benzodiazepine receptors in ethanol-induced increases of diazepam binding inhibitor (DBI) and its mRNA in the mouse brain

We have attempted to clarify the mechanisms for alcohol (EtOH)-induced elevation of diazepam binding inhibitor (DBI) mRNA and to investigate whether the increase in DBI mRNA is paralleled with that in DBI using EtOH-treated mice and primary cultured neurons. Both the DBI content and the expression o...

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Bibliographic Details
Published in:Brain research. Molecular brain research. 1998-02, Vol.54 (1), p.124-132
Main Authors: Katsura, Masashi, Ohkuma, Seitaro, Tsujimura, Atsushi, Xu, Jun, Hibino, Yasushi, Ishikawa, Emiko, Kuriyama, Kinya
Format: Article
Language:English
Subjects:
Administration, Inhalation
Alcoholism
Alcoholism and acute alcohol poisoning
Animals
Benzodiazepine (BDZ) receptor
Biological and medical sciences
Brain Chemistry - drug effects
Brain Chemistry - genetics
Carrier Proteins - drug effects
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cells, Cultured
Cerebral Cortex - cytology
Cerebral Cortex - drug effects
Cerebral Cortex - metabolism
DBI mRNA
Diazepam Binding Inhibitor
Diazepam binding inhibitor (DBI)
Ethanol - toxicity
Ethanol inhalation
Ethanol withdrawal
GABA-A Receptor Agonists
GABA-A Receptor Antagonists
Male
Medical sciences
Mice
Rabbits
Receptors, GABA-A - physiology
RNA, Messenger - biosynthesis
RNA, Messenger - metabolism
Toxicology
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Summary:We have attempted to clarify the mechanisms for alcohol (EtOH)-induced elevation of diazepam binding inhibitor (DBI) mRNA and to investigate whether the increase in DBI mRNA is paralleled with that in DBI using EtOH-treated mice and primary cultured neurons. Both the DBI content and the expression of DBI mRNA were elevated in the cerebral cortex of EtOH-inhaled and -withdrawn mice. Simultaneous administration of flunitrazepam (FLN) and Ro15-1788 with EtOH vapor completely abolished the EtOH-induced elevation of DBI mRNA. In addition, the exposure of the neurons for 3 days significantly elevated the expression of DBI mRNA, which was completely inhibited by concomitant exposure of FLN, Ro15-4513 and Ro15-1788 with EtOH, while muscimol and bicuculline showed no effects on the EtOH-induced increase of DBI mRNA expression. These results indicate that functional interaction between EtOH and benzodiazepine (BDZ) receptors is a critical role in the increased expression of DBI mRNA.
ISSN:0169-328X
1872-6941
DOI:10.1016/S0169-328X(97)00330-6