In Vitro Method of Studying the Angiotensin Activity of Chemical Compounds

We developed and tested an experimental model to study in vitro the type 1 angiotensin antagonistic activity of compounds on the isolated portal vein of rats. The reliability of this method was confirmed in tests with saralasin (nonselective antagonist of angiotensin receptors) and losartan (selecti...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2014-11, Vol.158 (1), p.115-117
Main Authors: Spasov, A. A., Yakovlev, D. S., Bukatina, T. M., Brigadirova, A. A.
Format: Article
Language:English
Subjects:
Angiotensin
Angiotensin II Type 1 Receptor Blockers - pharmacology
Animals
Biomedical and Life Sciences
Biomedicine
Cell Biology
Drug Evaluation, Preclinical
Female
In Vitro Techniques
Inhibitory Concentration 50
Internal Medicine
Laboratory Medicine
Losartan - pharmacology
Male
Methods
Muscle Contraction - drug effects
Muscle, Smooth, Vascular - drug effects
Pathology
Portal Vein - drug effects
Portal Vein - physiology
Rats, Wistar
Saralasin - pharmacology
Vasoconstriction - drug effects
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Summary:We developed and tested an experimental model to study in vitro the type 1 angiotensin antagonistic activity of compounds on the isolated portal vein of rats. The reliability of this method was confirmed in tests with saralasin (nonselective antagonist of angiotensin receptors) and losartan (selective antagonist of type 1 angiotensin receptors) in concentrations of 10 −9 -10 −5 mol/liter. The half-maximal inhibitory concentrations (IC 50 ) of these substances were calculated.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-014-2705-8