Discrimination between cultivars of Vitis vinifera based on molecular variability concerning 5' untranslated regions of the StSy-CHS genes

The degree of polymorphism present in 5' untranslated regions of stilbene synthase (StSy)-like loci was assessed. A ligation-mediated polymerase chain reaction (LM-PCR) cloning strategy was adopted to isolate sequences located immediately upstream of StSy coding regions. Among several clones, 1...

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Bibliographic Details
Published in:Theoretical and applied genetics 1997-08, Vol.95 (3), p.375-383
Main Authors: Geuna, F, Hartings, H, Scienza, A
Format: Article
Language:English
Subjects:
acyltransferases
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Cluster analysis
cultivar identification
Deoxyribonucleic acid
dessert cultivars
Discrimination
DNA
Fundamental and applied biological sciences. Psychology
genes
Genetic aspects
genetic polymorphism
Genetic polymorphisms
Genetics of eukaryotes. Biological and molecular evolution
Grapes
Identification and classification
microsatellite repeats
molecular sequence data
naringenin-chalcone synthase
nucleotide sequences
phylogeny
Plant genetics
Polymerase chain reaction
promoter regions
Pteridophyta, spermatophyta
repetitive sequences
stilbene synthase
stsy gene
Vegetals
Vitis vinifera
wine cultivars
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Summary:The degree of polymorphism present in 5' untranslated regions of stilbene synthase (StSy)-like loci was assessed. A ligation-mediated polymerase chain reaction (LM-PCR) cloning strategy was adopted to isolate sequences located immediately upstream of StSy coding regions. Among several clones, 13 randomly chosen fragments were analyzed at the sequence level. Four of the analyzed fragments appeared of particular interest. Two carried sequences reminiscent of micro-satellites, while the remaining fragments contained direct repeats. Oligonucleotides constructed against the specific DNA sequence of these clones disclosed a complex banding pattern when used in polymerase chain reaction (PCR)-analysis of 22 ancient varieties of grapevine. A total of 40 polymorphic bands could be identified and used to calculate coefficients of genetic similarity (GS) between varieties. GS values were used in cluster analysis to differentiate the 22 varieties. The data obtained are in good agreement with available information concerning the relationships between the varieties considered. This suggests the use of the method we have developed in fingerprinting studies of Vitis vinifera germ plasma.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220050573