Kinetic properties of cassava beta-glucosidase immobilized in photo-crosslinkable resins

Photo-crosslinkable resins (ENT-1000, ENT-2000 and ENT-4000) were used. Immobilized beta-glucosidase showed lower substrate affinity, better thermal stability, optimum pH range shifted towards the alkaline region and higher temperature optimum. beta-Glucosidase entrapped in ENT-4000 exhibited proper...

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Published in:Biotechnology letters 1991-01, Vol.13 (10), p.697-700
Main Author: Yeoh, H.H
Format: Article
Language:English
Subjects:
beta -glucosidase
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
heat stability
immobilization
Immobilization of enzymes and other molecules
Immobilization techniques
immobilized enzymes
kinetics
leaves
Manihot esculenta
Methods. Procedures. Technologies
physicochemical properties
resins
substrates
temperature
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description Photo-crosslinkable resins (ENT-1000, ENT-2000 and ENT-4000) were used. Immobilized beta-glucosidase showed lower substrate affinity, better thermal stability, optimum pH range shifted towards the alkaline region and higher temperature optimum. beta-Glucosidase entrapped in ENT-4000 exhibited properties closest to those of the free enzyme.
doi_str_mv 10.1007/BF01088171
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source Springer LINK Archives
subjects beta -glucosidase
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
heat stability
immobilization
Immobilization of enzymes and other molecules
Immobilization techniques
immobilized enzymes
kinetics
leaves
Manihot esculenta
Methods. Procedures. Technologies
physicochemical properties
resins
substrates
temperature
title Kinetic properties of cassava beta-glucosidase immobilized in photo-crosslinkable resins
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