Electrochemical Detection of Steroid Hormones Using a Nickel-Modified Glassy Carbon Electrode

This paper demonstrates the development of an analytical method for detecting steroid hormones by coupling HPLC to electrochemical detection, using a nickel‐modified glassy carbon electrode. The method was evaluated in terms of sensitivity, linear dynamic range, limit of detection, and response stab...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2014-10, Vol.26 (10), p.2145-2151
Main Authors: Muna, Grace W., Partridge, Michael, Sirhan, Hala, VerVaet, Bridget, Guerra, Nigel, Garner, Holly
Format: Article
Language:English
Subjects:
Dynamic range
Electrocatalytic oxidation
Electrochemical detection
Electrodes
Glassy carbon
Hormones
Joining
Mathematical analysis
Nickel
Nickel-modified glassy carbon electrode
Steroid hormones
Steroids
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Summary:This paper demonstrates the development of an analytical method for detecting steroid hormones by coupling HPLC to electrochemical detection, using a nickel‐modified glassy carbon electrode. The method was evaluated in terms of sensitivity, linear dynamic range, limit of detection, and response stability. The developed method exhibited good figures of merit for the steroid hormones studied with no evidence of electrode fouling. As an example, the limit of detection (S/N=3) for E3 was 0.10 µM and the response precision (n=5) was 0.6 %. The application of the method for the analysis of a real river water sample is demonstrated.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.201400361