The loss of CD34+ cells in peripheral hematopoietic stem cell products cryopreserved by non-controlled rate freezing and stored at −80 °C after overnight storage

• Published in: Transfusion and apheresis science 2014-10, Vol.51 (2), p.188-192
• Main Authors: Donmez, Ayhan, Yilmaz, Fergun, Soyer, Nur, Cagirgan, Seckin, Arik, Bahar, Tombuloglu, Murat
• Format: Article
• Language: English
• Subjects: Adult; Amyloidosis - blood; Amyloidosis - therapy; Antigens, CD34 - metabolism; Autografts; CD34+ cells; Cryopreservation; Female; Graft Survival; Health technology assessment; Hematologic Neoplasms - blood; Hematologic Neoplasms - therapy; Hematology, Oncology and Palliative Medicine; Hematopoietic Stem Cells - metabolism; Humans; Male; Middle Aged; Overnight storage; Peripheral blood stem cell; Peripheral Blood Stem Cell Transplantation; Prospective Studies; Time Factors
• ISSN: 1473-0502; 1878-1683
• DOI: 10.1016/j.transci.2014.08.013

Abstract Although peripheral blood stem cell (PBSC) products cryopreserved by non-controlled rate freezing and stored at −80 °C after overnight storage are used frequently, data regarding the rate of loss of CD34+ cells in these products are limited. In this prospective study, CD34+ cells were counted at three (fresh, post-overnight and post-thaw) points in 83 PBSC products from 41 patients by flow cytometry. Compared to fresh products, the mean losses of post-overnight and post-thaw total CD34+ cells are 16.3% and 38.4% ( p  = 0.02), and the mean losses of post-overnight and post-thaw viable CD34+ cells are 16.5% and 48.5%, respectively ( p