Modification of glassy carbon electrode surfaces with mediators and bridge molecules

Glassy carbon electrodes have been modified with the mediators di( n-aminopropyl)viologen (DAPV), p-benzoquinone (BQ), and with the bridge molecule enthylenediamine (EDA), by electrochemical oxidation of the electrode surface. The formation of reactive surface groups is probably involved. DAPV has b...

Full description

Saved in:
Bibliographic Details
Published in:Biosensors & bioelectronics 1991, Vol.6 (5), p.413-423
Main Authors: Hoogvliet, J.C., van Os, P.J.H.J., van der Mark, E.J., van Bennekom, W.P.
Format: Article
Language:English
Subjects:
Armoracia lapathifolia
Biological and medical sciences
Biosensors
Biotechnology
bridge molecule
carbon
chemical modification
Desulfovibrio vulgaris
di(n-aminopropyl)viologen
electrochemistry
electrode modification
electrodes
electron transfer
ethylenediamine
Fundamental and applied biological sciences. Psychology
glassy carbon
horseradish peroxidase
hydrogenase
mediator
Methods. Procedures. Technologies
p-benzoquinone
peroxidase
primary amines
quinone
surface groups
Various methods and equipments
viologen
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Glassy carbon electrodes have been modified with the mediators di( n-aminopropyl)viologen (DAPV), p-benzoquinone (BQ), and with the bridge molecule enthylenediamine (EDA), by electrochemical oxidation of the electrode surface. The formation of reactive surface groups is probably involved. DAPV has been used as a model compound for the attachment of substances containing a primary aliphatic amine group. BQ could be attached to the electrode directly or by coupling to an EDA-modified electrode. Noradrenaline and NADH were attached to the EDA-modified carbon surface using glutaraldehyde. Two enzymes, Desulfovibrio vulgaris hydrogenase and horseradish peroxidase (HRP) showed mediated electron transfer with the DAPV- and BQ-modified electrodes, respectively. Electrodes modified with BQ and HRP were used in the amperometric mode for the determination of hydrogen peroxide in a flow injection analysis system.
ISSN:0956-5663
1873-4235
DOI:10.1016/0956-5663(91)87006-W