The impact of nitric oxide on calcium homeostasis in PE/CA-PJ15 cells

Highlights • We investigated the effect of exogenous NO on Ca2+ homeostasis in PE/CA-PJ15 cells. • We found that NO generated a dose-dependent reduction in the cytosolic calcium concentration ([Ca2+ ]c ). • NO depletion using HbO2 as a scavenger, blocked any variation in the ([Ca2+ ]c ) induced by N...

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Bibliographic Details
Published in:Archives of oral biology 2014-12, Vol.59 (12), p.1377-1383
Main Authors: Tiribuzi, Roberto, Tartacca, Francesco, Aisa, Maria Cristina, Cerulli, Giuliano Giorgio, Palmerini, Carlo Alberto
Format: Article
Language:English
Subjects:
Advanced Basic Science
Calcium Signaling - drug effects
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology
Cell Survival - drug effects
Cysteine - analogs & derivatives
Cysteine - pharmacology
Cytosol - chemistry
Cytosolic calcium
Dentistry
Dose-Response Relationship, Drug
Endoplasmic reticulum
Fura-2 - pharmacology
Homeostasis - drug effects
Humans
Mitochondria
Mitochondrion
Nitric oxide
Nitric Oxide - pharmacology
Oligomycins - pharmacology
Oxyhemoglobins - pharmacology
S-Nitrosothiols - pharmacology
Thapsigargin - pharmacology
Tumor Cells, Cultured
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Summary:Highlights • We investigated the effect of exogenous NO on Ca2+ homeostasis in PE/CA-PJ15 cells. • We found that NO generated a dose-dependent reduction in the cytosolic calcium concentration ([Ca2+ ]c ). • NO depletion using HbO2 as a scavenger, blocked any variation in the ([Ca2+ ]c ) induced by NO. • The inhibition of mitochondrial respiration with FCCP and oligomycin strongly reduced the [Ca2+ ]c decrease induced by NO. • During the mitochondrion energy deficit, the NO effect on ([Ca2+ ]c ) is also reduced in the presence of La3+ and thapsigargin.
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2014.07.022