Graphene/TiO2 nanocomposite based solid-phase extraction and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for lipidomic profiling of avocado (Persea americana Mill.)

• Published in: Analytica chimica acta 2014-12, Vol.852, p.153-161
• Main Authors: Shen, Qing, Yang, Mei, Li, Linqiu, Cheung, Hon-Yeung
• Format: Article
• Language: English
• Subjects: Avocado; Graphene/TiO2; Graphite - chemistry; Lipidomics; MALDI-TOF/MS; Nanocomposites - chemistry; Persea - chemistry; Phospholipids - analysis; Phospholipids - isolation & purification; Principal component analysis; Solid Phase Extraction - methods; Solid-phase extraction; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Titanium - chemistry
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2014.09.022

[Display omitted] •A SPE method using G/TiO2 as sorbent was developed.•This method can selectively extract phospholipids from avocado.•The analytes were detected by MALDI-TOF/MS in positive and negative modes.•This method is efficient and promising. Phospholipids possess important physiological, structural and nutritional functions in biological systems. This study described a solid-phase extraction (SPE) method, employing graphene and titanium dioxide (G/TiO2) nanocomposite as sorbent, for the selective isolation and enrichment of phospholipids from avocado (Persea americana Mill.). Based on the principal that the phosphoryl group in the phospholipid can interact with TiO2 via a bridging bidentate mode, an optimum condition was established for SPE, and was successfully applied to prepare avocado samples. The extracts were monitored by matrix-assisted laser desorption ionization time-of-flight/tandem mass spectrometry (MALDI-TOF/MS) in both positive-ion and negative-ion modes. Results showed that phospholipids could be efficiently extracted in a clean manner by G/TiO2 based SPE. In addition, the signals of phospholipids were enhanced while the noise was reduced. Some minor peaks became more obvious. In conclusion, the nanocomposite material of G/TiO2 was proved to be a promising sorbent for selective separation of phospholipids from crude lipid extract.