Estimation of skin concentrations of topically applied lidocaine at each depth profile

[Display omitted] Skin concentrations of topically administered compounds need to be considered in order to evaluate their efficacies and toxicities. This study investigated the relationship between the skin permeation and concentrations of compounds, and also predicted the skin concentrations of th...

Full description

Saved in:
Bibliographic Details
Published in:International journal of pharmaceutics 2014-11, Vol.475 (1-2), p.292-297
Main Authors: Oshizaka, Takeshi, Kikuchi, Keisuke, Kadhum, Wesam R., Todo, Hiroaki, Hatanaka, Tomomi, Wierzba, Konstanty, Sugibayashi, Kenji
Format: Article
Language:English
Subjects:
Administration, Topical
Animals
Concentration-distance profile
Dermis - metabolism
Diffusion
Epidermis - metabolism
Lidocaine - chemistry
Lidocaine - metabolism
Permeability
Permeation parameter
Skin Absorption
Skin concentration
Skin permeation
Solutions - chemistry
Solutions - metabolism
Swine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] Skin concentrations of topically administered compounds need to be considered in order to evaluate their efficacies and toxicities. This study investigated the relationship between the skin permeation and concentrations of compounds, and also predicted the skin concentrations of these compounds using their permeation parameters. Full-thickness skin or stripped skin from pig ears was set on a vertical-type diffusion cell, and lidocaine (LID) solution was applied to the stratum corneum (SC) in order to determine in vitro skin permeability. Permeation parameters were obtained based on Fick's second law of diffusion. LID concentrations at each depth of the SC were measured using tape-stripping. Concentration-depth profiles were obtained from viable epidermis and dermis (VED) by analyzing horizontal sections. The corresponding skin concentration at each depth was calculated based on Fick's law using permeation parameters and then compared with the observed value. The steady state LID concentrations decreased linearly as the site became deeper in SC or VED. The calculated concentration-depth profiles of the SC and VED were almost identical to the observed profiles. The compound concentration at each depth could be easily predicted in the skin using diffusion equations and skin permeation data. Thus, this method was considered to be useful for promoting the efficient preparation of topically applied drugs and cosmetics.
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2014.08.046