Antioxidant and antibacterial activities of exopolysaccharides from Bifidobacterium bifidum WBIN03 and Lactobacillus plantarum R315

• Published in: Journal of dairy science 2014-12, Vol.97 (12), p.7334-7343
• Main Authors: Li, Shengjie, Huang, Renhui, Shah, Nagendra P., Tao, Xueying, Xiong, Yonghua, Wei, Hua
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Anti-Infective Agents; Antioxidants - pharmacology; antioxidative and antibacterial activity; Ascorbic Acid - pharmacology; Bifidobacterium - chemistry; Bifidobacterium bifidum; Biphenyl Compounds; exopolysaccharide; Lactobacillus plantarum; Lactobacillus plantarum - chemistry; Lipid Peroxidation - drug effects; Oxidation-Reduction; Picrates; Polysaccharides, Bacterial - pharmacology
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2014-7912

The objective of this study was to investigate the antioxidant and antibacterial activities of exopolysaccharide (EPS) from Bifidobacterium bifidum WBIN03 (B-EPS) and Lactobacillus plantarum R315 (L-EPS). The 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging, hydroxyl radical-scavenging, and superoxide radical-scavenging abilities were measured to evaluate antioxidant activity. Inhibition of erythrocyte hemolysis and lipid peroxidation was also measured. Both B-EPS and L-EPS had strong scavenging ability against DPPH and superoxide radicals at high concentration. The inhibitory effect of B-EPS on erythrocyte hemolysis was stronger than that of L-EPS in a concentration range from 0.30 to 1.00mg/mL, whereas the hydroxyl scavenging ability of L-EPS (39.15±0.58%) was significantly higher than that of 0.15mg/mL ascorbic acid (24.33±1.17%) and B-EPS (17.89±3.30%) at 0.10mg/mL. The inhibition of lipid peroxidation of 0.50mg/mL B-EPS and L-EPS was 13.48±1.74% and 12.43±0.51%, respectively, values lower than that of ascorbic acid at the same concentration (23.20±1.41%). Furthermore, all these abilities were enhanced in a concentration-dependent manner. Agar diffusion assay showed that both EPS exhibited antibacterial activities against tested pathogens such as Cronobacter sakazakii, Escherichia coli, Listeria monocytogenes, Staphyloccocus aureus, Candida albicans, Bacillus cereus, Salmonella typhimurium, and Shigella sonnei at 300μg/mL. In conclusion, both EPS have antimicrobial and antioxidant activities and could have applications in the food industry.