Antioxidant and antityrosinase proanthocyanidins from Polyalthia longifolia leaves

• Published in: Journal of bioscience and bioengineering 2014-11, Vol.118 (5), p.583-587
• Main Authors: Chen, Xiao-Xin, Liang, Ge, Chai, Wei-Ming, Feng, Hui-Ling, Zhou, Han-Tao, Shi, Yan, Chen, Qing-Xi
• Format: Article
• Language: English
• Subjects: Antioxidant activity; Antioxidants - chemistry; Antioxidants - isolation & purification; Antityrosinase activity; Ascorbic Acid - chemistry; Benzothiazoles - chemistry; Biflavonoids - chemistry; Biflavonoids - isolation & purification; Biological and medical sciences; Biotechnology; Biphenyl Compounds - chemistry; Biphenyl Compounds - isolation & purification; Catechin - chemistry; Catechin - isolation & purification; Chromatography, High Pressure Liquid; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - isolation & purification; Enzyme Inhibitors - pharmacology; Free Radical Scavengers - chemistry; Fundamental and applied biological sciences. Psychology; Inhibitory Concentration 50; Magnetic Resonance Spectroscopy; Monophenol Monooxygenase - antagonists & inhibitors; Oxidation-Reduction; Picrates - chemistry; Picrates - isolation & purification; Plant Leaves - chemistry; Polyalthia - chemistry; Polyalthia longifolia; Proanthocyanidins; Proanthocyanidins - chemistry; Proanthocyanidins - isolation & purification; Proanthocyanidins - pharmacology; Reducing Agents - chemistry; Spectrometry, Mass, Electrospray Ionization; Structure characterization; Sulfonic Acids - chemistry
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2014.04.015

In the present study the structure of proanthocyanidins from Polyalthia longifolia leaves was characterized with 13C nuclear magnetic resonance, high performance liquid chromatography electrospray ionization mass spectrometry, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses. The results showed that the proanthocyanidins were mixture of homopolymers of B-type procyanidins with degree of polymerization up to 14-mer. Furthermore, the antioxidant activity of the proanthocyanidins was studied through 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free-radical scavenging activities, and ferric reducing/antioxidant power assays. In addition, antityrosinase activity of the proanthocyanidins was investigated. The IC50 for 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free-radical scavenging activity of the proanthocyanidins were 89.32 ± 12.07 and 76.79 ± 5.88 μg/mL, respectively; the ferric reducing/antioxidant power value was 710.54 ± 142.82 mg ascorbic acid equivalent/g dry weight. The IC50 for antityrosinase activity was 773.09 ± 1.47 μg/mL. In conclusion, the proanthocyanidins from P. longifolia leaves exhibited potent antioxidant and antityrosinase activities. This research would provide scientific evidence for the use of proanthocyanidins from P. longifolia leaves as antioxidant and antityrosinase agents.