Structural characterization, sulfation and antitumor activity of a polysaccharide fraction from Cyclina sinensis

•Structural characterization of CSPS-1 was performed by methylation and GC–MS.•Backbone chains of CSPS-1 were linked by 1→4 glycosidic bonds.•Branches were attached to main chain by 1→6 glycosidic bonds.•Sulfated modification of CSPS-1 was accomplished by the CSA-Pyr method.•Antitumor activity of CS...

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Bibliographic Details
Published in:Carbohydrate polymers 2015-01, Vol.115, p.200-206
Main Authors: Jiang, Changxing, Xiong, Qingping, Li, Songlin, Zhao, Xirong, Zeng, Xiaoxiong
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacology
Antitumor activity
Bivalvia - chemistry
Cell Line, Tumor
Cyclina sinensis
Humans
Polysaccharide
Polysaccharides - chemistry
Polysaccharides - pharmacology
Structural characterization
Sulfates - chemistry
Sulfation
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Summary:•Structural characterization of CSPS-1 was performed by methylation and GC–MS.•Backbone chains of CSPS-1 were linked by 1→4 glycosidic bonds.•Branches were attached to main chain by 1→6 glycosidic bonds.•Sulfated modification of CSPS-1 was accomplished by the CSA-Pyr method.•Antitumor activity of CSPS-1 was enhanced after sulfated modification. In the present study, we investigated the preliminary structure, sulfation and antitumor activity of a polysaccharide fraction from Cyclina sinensis (CSPS-1). Results of structural characterization showed that the backbone chain of CSPS-1 was composed of glucose linked by α-(1→4) glycosidic bond, and the branch chain was attached to backbone chain by (1→6) glycosidic bond. CSPS-1 was sulfated by chlorosulfonic acid-pyridine method under different modification conditions according to the orthogonal test L9(34), affording nine sulfated polysaccharides (CSPS-1-S). The optimal sulfation conditions for CSPS-1 were reaction temperature of 65°C, reaction time of 2h and chlorosulfonic acid-pyridine ratio of 1:4. Structural analysis revealed that sulfation had occurred at position of C-6 in CSPS-1. In addition, CSPS-1-S exhibited significantly higher inhibitory activity in vitro against human gastric cancer BGC-823 cells.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2014.08.095