Cytotoxicity testing of silver-containing burn treatments using primary and immortal skin cells

Abstract A novel burn wound hydrogel dressing has been previously developed which is composed of 2-acrylamido-2-methylpropane sulfonic acid sodium salt with silver nanoparticles (silver AMPS). This study compared the cytotoxicity of this dressing to the commercially available silver products; Actico...

Full description

Saved in:
Bibliographic Details
Published in:Burns 2014-12, Vol.40 (8), p.1562-1569
Main Authors: Boonkaew, Benjawan, Kempf, Margit, Kimble, Roy, Cuttle, Leila
Format: Article
Language:English
Subjects:
Acrylamides - pharmacology
Alkanesulfonates - pharmacology
Bandages, Hydrocolloid
Burn care
Burns - therapy
Cell Survival - drug effects
Critical Care
Cytotoxicity
Dressing
Fibroblasts - drug effects
Glycerol - pharmacology
Humans
Hydrogel
Keratinocytes - drug effects
Metal Nanoparticles
Polyesters - pharmacology
Polyethylenes - pharmacology
Polyurethanes - pharmacology
Silver - pharmacology
Silver nanoparticles
Silver Sulfadiazine - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract A novel burn wound hydrogel dressing has been previously developed which is composed of 2-acrylamido-2-methylpropane sulfonic acid sodium salt with silver nanoparticles (silver AMPS). This study compared the cytotoxicity of this dressing to the commercially available silver products; Acticoat™, PolyMem Silver® and Flamazine™ cream. Human keratinocytes (HaCaT and primary HEK) and normal human fibroblasts (NHF) were exposed to dressings incubated on Nunc™ polycarbonate inserts for 24, 48 and 72 h. Four different cytotoxicity assays were performed including; Trypan Blue cell count, MTT, Celltiter-Blue™ and Toluidine Blue surface area assays. The results were expressed as relative cell viability compared to an untreated control. The cytotoxic effects of Acticoat™ and Flamazine™ cream were dependent on exposure time and cell type. After 24 h exposure, Acticoat™ and Flamazine™ cream were toxic to all tested cell lines. Surprisingly, HaCaTs treated with Acticoat™ and Flamazine™ had an improved ability to survive at 48 and 72 h while HEKs and NHFs had no improvement in survival with any treatment. The novel silver hydrogel and PolyMem Silver® showed low cytotoxicity to all tested cell lines at every time interval and these results support the possibility of using the novel silver hydrogel as a burn wound dressing. Researchers who rely on HaCaT cells as an accurate keratinocyte model should be aware that they can respond differently to primary skin cells.
ISSN:0305-4179
1879-1409
DOI:10.1016/j.burns.2014.02.009