Comparison of meniscal fibrochondrocyte and synoviocyte bioscaffolds toward meniscal tissue engineering in the dog

Tissue engineering is a promising field of study toward curing the meniscal deficient stifle; however the ideal cell type for this task is not known. We describe here the extraction of synoviocytes and meniscal fibrochondrocytes from arthroscopic debris from six dogs, which were cultured as tensione...

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Bibliographic Details
Published in:Research in veterinary science 2014-10, Vol.97 (2), p.400-408
Main Authors: Ballard, George A., Warnock, Jennifer J., Bobe, Gerd, Duesterdieck-Zellmer, Katja F., Baker, Lindsay, Baltzer, Wendy I., Ott, Jesse
Format: Article
Language:English
Subjects:
Animals
Biomechanics
Cell culture
Cell Survival - physiology
Cells, Cultured
Collagen
Collagen - metabolism
Cytokines
Dogs - injuries
Dogs - surgery
Extracellular matrix
Female
Fibrocartilage - cytology
Fibrocartilage - metabolism
Gene expression
Glycosaminoglycans - metabolism
Hypotheses
Male
Menisci, Tibial - surgery
Meniscus
Osteoarthritis
Stem cells
Synovial Membrane - cytology
Synovial Membrane - metabolism
Synovium
Tibial Meniscus Injuries
Tissue engineering
Tissue Engineering - methods
Tissue Engineering - veterinary
Tissue Scaffolds - veterinary
Transplants & implants
Veterinary medicine
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Summary:Tissue engineering is a promising field of study toward curing the meniscal deficient stifle; however the ideal cell type for this task is not known. We describe here the extraction of synoviocytes and meniscal fibrochondrocytes from arthroscopic debris from six dogs, which were cultured as tensioned bioscaffolds to synthesize meniscal-like fibrocartilage sheets. Despite the diseased status of the original tissues, synoviocytes and meniscal fibrochondrocytes had high viability at the time of removal from the joint. Glycosaminoglycan and collagen content of bioscaffolds did not differ. Meniscal fibrochondrocyte bioscaffolds contained more type II collagen, but collagen deposition was disorganized, with only 30–40% of cells viable. The collagen of synoviocyte bioscaffolds was organized into sheets and bands and 80–90% of cells were viable. Autologous, diseased meniscal fibrochondrocytes and synoviocytes are plausible cell sources for future meniscal tissue engineering research, however cell viability of meniscal fibrochondrocytes in the tensioned bioscaffolds was low.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2014.05.002