Reduction of Proliferative Heterogeneity of CHEF18 Chinese Hamster Cell Line during the Progression toward Tumorigenicity

Doubling time and generation time represent two parameters by which the proliferation of cultured mammalian cells can be monitored. In this study we report the characterization of CHEF 18 Chinese hamster cell line during the progression toward tumorigenicity by analysis of doubling time and generati...

Full description

Saved in:
Bibliographic Details
Published in:In Vitro Cellular & Developmental Biology - Animal 1991-12, Vol.27A (12), p.949-952
Main Authors: RAINALDI, G, PINTO, B, PIRAS, A, VATTERONI, L, SIMI, S, CITTI, L
Format: Article
Language:English
Subjects:
3T3 cells
Animal cells
Animals
Biological and medical sciences
Cell cycle
Cell cycle, cell proliferation
Cell Division - physiology
Cell growth
Cell lines
Cell physiology
Cell Transformation, Neoplastic - genetics
Cell Transformation, Neoplastic - pathology
characterization
CHO cells
Chromatids
Cricetinae
Cricetulus
Cultured cells
Doubling time
Fibroblasts - cytology
Fibroblasts - physiology
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
G1 Phase
Genetic Variation - genetics
Interphase
Molecular and cellular biology
Time Factors
Tumor cell line
tumorigenicity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Doubling time and generation time represent two parameters by which the proliferation of cultured mammalian cells can be monitored. In this study we report the characterization of CHEF 18 Chinese hamster cell line during the progression toward tumorigenicity by analysis of doubling time and generation time. The two parameters reveal that the proliferation was initially different, indicating the presence of a proliferative heterogeneity among the cycling cells. The progressive reduction up to the disappearance of this discrepancy suggests that a modification of the length of some phases of the cell cycle may have occurred during the progression toward tumorigenicity. However, the hypothesis that the shortening of doubling time might be due to a continuous coming out of cells from the cell cycle rather than to a shortening of the cell cycle is presented.
ISSN:0883-8364
2327-431X
1543-706X
DOI:10.1007/BF02631122