Determination of alpha-amylase activity in dextran, Ficoll and polyethylene glycol solutions

A new insoluble chromolytic substrate for the spectrophotometric determination of alpha-amylase activity (starch cross-linked with 1,4-butanediol diglycidyl ether in the presence of black drawing ink; "black starch") has been shown to work well also in the presence of dextran, Ficoll and p...

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Bibliographic Details
Published in:Biotechnology techniques 1992-03, Vol.6 (2), p.177-180
Main Authors: Safarik, I, Safarikova, M
Format: Article
Language:English
Subjects:
activity
alpha -amylase
Analytical, structural and metabolic biochemistry
assays
Bacillus (bacteria)
Bacillus licheniformis
Biological and medical sciences
black starch
corn starch
dextran
dyes
enzyme activity
Enzymes and enzyme inhibitors
ficoll
food biotechnology
Fundamental and applied biological sciences. Psychology
Hydrolases
hydrolysis
ink
polyethylene glycol
polymers
spectrophotometry
spectroscopy
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Summary:A new insoluble chromolytic substrate for the spectrophotometric determination of alpha-amylase activity (starch cross-linked with 1,4-butanediol diglycidyl ether in the presence of black drawing ink; "black starch") has been shown to work well also in the presence of dextran, Ficoll and polyethylene glycol; on the other hand these phase-forming polymers interfere with some commonly used alpha-amylase assays.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF02438828