Identification of minor fimbrianl subunits involved in biosynthesis of K88 fimbriae

The nucleotide sequences of the genes faeF, faeH, faeI, and faeJ encoding K88 minor fimbrial subunits were determined. Analysis of the primary structure of the gene products revealed that all four proteins are synthesized with an amino-terminal signal sequence. The molecular masses of the mature Fae...

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Published in:Journal of bacteriology 1992-01, Vol.174 (20), p.6350-6358
Main Authors: Bakker, D, Willemsen, PTJ, Willems, R H, Huisman, T T, Mooi, FR, Oudega, B, Stegehuis, F, de Graaf, FK
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Escherichia coli
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container_issue 20
container_start_page 6350
container_title Journal of bacteriology
container_volume 174
creator Bakker, D
Willemsen, PTJ
Willems, R H
Huisman, T T
Mooi, FR
Oudega, B
Stegehuis, F
de Graaf, FK
description The nucleotide sequences of the genes faeF, faeH, faeI, and faeJ encoding K88 minor fimbrial subunits were determined. Analysis of the primary structure of the gene products revealed that all four proteins are synthesized with an amino-terminal signal sequence. The molecular masses of the mature FaeF, FaeH, FaeI, and FaeJ proteins were calculated to be 15,161, 25,461, 24,804, and 25,093 Da, respectively. FaeH, FaeI, and FaeJ showed significant homology with FaeG, the major fimbrial subunit of K88 fimbriae. Mutations in the respective genes were constructed. Analysis of the mutants showed that the minor fimbrial subunits FaeF and FaeH play an essential role in the biogenesis but not in the adhesive properties of the K88 fimbriae. Mutations in faeI or faeJ had no significant effect on K88 production or adhesive capacity. Immunoblotting and immunoelectron microscopy revealed that FaeF and FaeH are located in or along the K88 fimbrial structure.
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title Identification of minor fimbrianl subunits involved in biosynthesis of K88 fimbriae
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