Application of marker-assisted selection in rice for bacterial blight resistance gene, Xa21

Polymerase chain reaction (PCR)-based marker-assisted selection for a disease resistance gene was employed in a rice improvement programme. PCR amplification using sequence-tagged site (STS) primers specific to bacterial blight resistance gene Xa21-linked marker resulted in amplified products of the...

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Bibliographic Details
Published in:Current science (Bangalore) 1997-11, Vol.73 (10), p.873-875
Main Authors: Reddy, J. N., Baraoidan, M. R., Bernardo, M. A., George, M. L. C., Sridhar, R.
Format: Article
Language:English
Subjects:
Bacterial diseases
Blight
Disease resistance
Mendelian inheritance
Molecular weight
Plant breeding
Plants
Polymerase chain reaction
RESEARCH COMMUNICATIONS
Rice
Sequence tagged sites
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Summary:Polymerase chain reaction (PCR)-based marker-assisted selection for a disease resistance gene was employed in a rice improvement programme. PCR amplification using sequence-tagged site (STS) primers specific to bacterial blight resistance gene Xa21-linked marker resulted in amplified products of the expected molecular weights for each of the three genotypic classes, homozygous resistant, homozygous susceptible and heterozygous and facilitated selection of plants carrying the desired gene for further advancing the breeding material.
ISSN:0011-3891